cloning

Sickle-cell anemia arises from a mutation in the gene for the β chain of human haemoglobin. The change from GAG to GTG in the mutant eliminates a cleavage site for the restriction enzyme MstII, which recognizes the target sequence CCTGAGG. In the figure below MstII sites are indicated by vertical lines and the size of DNA fragments represented by horizontal double-arrows is indicated in kilobasepairs (kbp). These findings form the basis of a diagnostic test for the sickle-cell gene.:

a) Propose two methods - based on DNA analysis - for distinguishing between the normal and mutant gene
b) What would be the result of these analyses on DNA from a normal person and from a person carrying the sickle-cell gene?