Hjælp til at besvare 7 multiple choice

Hej er der nogle der kan hjælpe med nedenstående spørgsmål. Jeg har skrevet mine svar nederst, men jeg er usikker på om mine svar er rigtige. 

1) PCR can be used to amplify a specific sequense of a gene while RT-PCR is a variant of PCR used for analysis of gene expression. Which of the following is/are not used for PCR?

- DNA

- downstream primer 

- mRNA

- upstream primer 

- deoxynucleoside triphosphates 

- reverse transcriptase

- Taq polymerase 

- restriction enzyme

2) A PCR reaction consists of 3 steps: 

- denaturation, primer annealing and elongation

- primer annealing, elongation and temination

- Initiation, elongation and temination

- denaturation, initiation and elongation 

3) A PCR reaction that continues for 30 cycles will produce approximately -- PCR products from a single template DNA molecule 

- 60 

- 900 

- 1.000.000

- 1.000.000.000

4) Restriction enzyme is about restriction enzymes. Which of the following is/are true?
- Restriction enzymes are endonucleases

- Restriction enzyme cleave DNA sequences randomly 

- Restriction enzymes are exonucleases

- Restriction enzymes recognize and cleave specifik sequences about 4-8 base pairs long 

5) MNase, Which of the following is/are true?

- MNase is a endonuclease 

- MNase cleaves DNA sequenses randomly 

- MNase recognizes and cleaves specifik sequences about 4-8 base pairs long 

- MNase is a exonuclease

6) Which of the statements about gel electrophoresis is/are true? 

- Gel electrophoresis can be used to seperate DNA and RNA of different size

- Molecular size of DNA and agarose gel concentration determines the rate by which DNA migrate through the gel 

- Gel elctrophoresis can be used to separate proteins of different size

7) Which of the following statement about SDS-page is/are true 

- SDS gel electrophoresis makes use of the migration of proteins in an electric field

- in an SDS-PAGE experiment proteins are seperated on the basis of their molecular weight

- In a SDS-PAGE gel proteins have - close to- the same charge - to - mass - ratio

- Proteins are solubilized but not denatured when seperated by SDS polyacrylamise gel electrophoresis

Mine forslag til svar: 

1) - DNA

2) - denaturation, primer annealing and elongation

3) - 1.000.000.000

4) - Restriction enzyme cleave DNA sequences randomly 

5) - MNase is a endonuclease 

6) - Gel electrophoresis can be used to seperate DNA and RNA of different size

7) Proteins are solubilized but not denatured when seperated by SDS polyacrylamise gel electrophoresis

På forhånd tak